http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105087516-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1241 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8202 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8261 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2015-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105087516-B |
| titleOfInvention | ADP-glucose pyrophosphorylase mutant and screening method and application thereof |
| abstract | The invention provides an ADP-glucose pyrophosphorylase mutant and a screening method and application thereof, wherein the screening method comprises the following steps: 1) synthesizing first strand cDNA; 2) PCR amplification; 3) constructing an AGPase subunit cDNA prokaryotic expression vector; 4) and (4) activity screening. The invention carries out distant hybridization on AGPase large subunit genes and small subunit genes from different species, and obtains an expression vector of a recombinant gene with high controllability; then transforming the escherichia coli glgC mutant strain, inoculating the strain on a Conberg enriched solid culture medium, and finally, using iodine-potassium iodide to dye and screen out the high-activity ADP-glucose pyrophosphorylase mutant; the ADP-glucose pyrophosphorylase mutant has enzyme activity and glycogen content obviously higher than those of wild corn AGPase, does not obviously change substrate affinity, and increases sensitivity to an activator. |
| priorityDate | 2015-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 581.