http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105087453-B
Outgoing Links
Predicate | Object |
---|---|
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate | 2014-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105087453-B |
titleOfInvention | Genetic engineering bacterium for biocatalysis flavone compound glucuronic acid glycosidation |
abstract | The present invention relates to genetically engineered biologicals, especially microorganism such as Escherichia coli, have the activity of catalysis flavone compound glucuronic acid glycosidation.The genetic engineering bacterium that flavone compound glucuronic acid glycosidation can be catalyzed co-expresses 4 genes for being separately encoded phosphoglucomutase, UDPglucose pyrophosphorylase, UDP-glucose dehydrogenase and uridine diphosphate glucuronatetransferase in the cell;These function enzyme genes import cell by expression vector and obtain genetic engineering bacterium.Engineered strain of the invention is the expression of the inducing function zymoprotein in the case where adding inducer isopropylthio thiogalactoside (IPTG), the glucuronic acid glycosidation of direct utilizing works bacterium biocatalysis flavone compound, cell well-grown simultaneously, fermentation period is shorter, at low cost. |
priorityDate | 2014-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 258.