http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105085606-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2015-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105085606-B |
| titleOfInvention | A kind of method using copper chelating Beads enrichment copper protein group |
| abstract | A kind of method using copper chelating Beads enrichment copper protein group is related to protein chemistry domain technology field；It takes biological tissue or after cell is fully ground with liquid nitrogen, adds in protein extract, after mixing, low-temperature centrifugation obtains the protein solution of denaturation；Copper is chelated magnetic bead to mix with the protein solution being denatured, is incubated, obtains magnetic bead protein mixed solution；The buffer solution 1 that 1 times of its volume is added in magnetic bead protein mixed solution is suspended, and the supernatant of half is removed under externally-applied magnetic field；It repeats 45 times, obtains mixed liquor A；The buffer solution 2 of 1 times of its volume is added in mixed liquor A, by all specific copper-binding proteins, i.e. copper protein group is separated, and copper protein group is collected under externally-applied magnetic field；The present invention separates the metalloprotein group under Denaturing using magnetic bead IDA, enormously simplifies program, improves the acquisition efficiency of metalloprotein, establishes a set of separation method for being suitable for metalloprotein group in excessive heavy metal treatment conditions undertissue or cell. |
| priorityDate | 2015-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.