http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105062956-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0622 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-062 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2015-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105062956-B |
| titleOfInvention | People's olfactory mucosa Olfactory essheathing cell is separated, passed on, freezing, differentiation technique |
| abstract | The invention provides the culture of people's olfactory mucosa Olfactory essheathing cell, pass on, freeze, differentiation technique.Characteristic step includes：1st, olfactory mucosa Olfactory essheathing cell culture medium is prepared with containing neurotrophic factors such as DMEM/DF12；2nd, the acquisition of schneiderian membrane；3rd, double enzyme combination digestion.4th, original cuiture：Cell suspension made of olfactory mucosa Olfactory essheathing cell culture medium is added, transfer cell is into blake bottle, in CO2 constant temperature and humidity incubator cultures；5th, Secondary Culture：Filtering medium supernatant is collected, prepares Secondary Culture base；6th, Cryopreservation：Filtering medium supernatant is collected, prepares special frozen stock solution；7th, cultivated using preceding differentiation.This patent chooses people's supraturbinal regio olfactoria mucous membrane and carries out culture propagation, pass on, freeze, breaking up, high-purity olfactory mucosa Olfactory essheathing cell can be obtained, the cell has global neurological repair, this cell of clinical treatment studies have shown that can effectively repair the nervous function of sacred disease and infringement forfeiture and improve the life quality of patient. |
| priorityDate | 2015-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.