http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105039519-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2015-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105039519-B |
titleOfInvention | A kind of DNA based on the hybridization of foot point domain determines new method |
abstract | A kind of DNA based on the hybridization of foot point domain determines new method, belongs to chemiluminescence field.Target dna is added in DNA1 modification magnetic beads, one end of target dna and DNA1 one end single stranded portion hybridize to form foot point domain, the non-hybridized part of target dna continues hybridization reaction with DNA1 under being acted in foot point domain, DNA1 hairpin structure is opened, form double-stranded DNA simultaneously, add the probe of FITC marker DNAs 2, one end single stranded portion of DNA2 one end and the double-stranded DNA formed hybridizes to form foot point domain, part non-hybridized DNA2 continues hybridization reaction with double-stranded DNA in the presence of foot point domain, form new double-stranded DNA, target dna is replaced and recycled, and substantial amounts of DNA2 is attached on magnetic bead, the measure to target dna is realized using optical diode induced chemical principle of luminosity.The inventive method is simple, cost is low, high sensitivity. |
priorityDate | 2015-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.