http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105039439-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-245 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-64 |
| filingDate | 2015-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105039439-B |
| titleOfInvention | It is coated the method for thalline biosynthesis conjugated linoleic acid isomers in organic media |
| abstract | The method of thalline biosynthesis conjugated linoleic acid isomers in organic media is coated, is included the following steps：（1）Every gram of 1.574 thalline of Lactobacillus casei CGMCC is resuspended in 5mL permeabilized treatment liquid, permeability thalline is collected by centrifugation in 37 DEG C of processing 20min；（2）After being washed with the phosphate buffer of 50mM, pH5.8, thalline were collected by centrifugation, every gram of permeability wet thallus is resuspended in the phosphate buffer of 20mL50mM, pH5.8, the polysorbas20 for adding in bacteria suspension volume 0.5 2.0% is uniformly mixed, 4 40 DEG C of 2 6h of processing, thalline were collected by centrifugation, and coating thalline is obtained after freeze-drying；（3）Every gram of coating thalline is added in 600mL n-hexanes, is stirred evenly, the linoleic acid of n-hexane volume 0.1 0.3% is added in, reacts 1 12h at 4 40 DEG C；（4）Coating thalline is centrifuged, recycles n-hexane, product is c 9, t 11 conjugated linoleic acid isomers.Reaction time of the invention is short, and coating thalline is reusable repeatedly, and yield is significantly improved, and non-environmental-pollution significantly reduces production cost. |
| priorityDate | 2015-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.