http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105039375-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2015-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105039375-B |
| titleOfInvention | A method of cloning vector is constructed using protein I HF |
| abstract | The invention discloses a kind of methods using protein I HF building cloning vector, by the DNA fragmentation design primer for treating connection, and it introduces DNA homology and organizes sequence together, it is being expanded respectively, amplified production and IHF mixing, it is converted and is cloned in Escherichia coli using traditional method for transformation, obtain recombinant dna plasmid.The method of the present invention is not limited independent of any recombinase by the restriction enzyme site of carrier and target fragment, completes the gene clone technology that target fragment is connected with carrier together with the method for homologous recombination and IHF albumen.The technical operation is simple, and one step directed cloning of PCR product, target fragment is connect with carrier homologous recombination, and the normal-temperature reaction time is as short as 10 minutes, and positive rate is up to 95% or more. |
| priorityDate | 2015-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.