http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105039343-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 |
| filingDate | 2015-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105039343-B |
| titleOfInvention | It is a kind of inhibit chicken cell cyclin F gene expressions shRNA molecule sequence and its application |
| abstract | The present invention relates to the shRNA slow virus interference carriers for inhibiting the gene expression efficiency best for chicken cell cyclin F genes, structure.For 4 shRNA oligonucleotide sequences of chicken cell cyclin F genes design, it is cloned into slow virus interference carrier lentiviral vector, construct four interference carriers, with packaging plasmid cotransfection 293T cells, viral suspension is arrived after the filtering of supernatant culture solution, with certain MOI virus quantities infected chicken embryo fibroblast and chicken embryo sustentacular cell of testis after measurement virus titer, the shRNA interference carriers of optimal inhibition efficiency are screened.Prove that it effectively reduces the expression of chicken cell cyclin F genes by real-time fluorescence quantitative PCR on molecular biology. |
| priorityDate | 2015-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.