http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105039243-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-073 |
| filingDate | 2015-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105039243-B |
| titleOfInvention | A kind of isolated culture method of chicken embryo arteria pulmonalis smooth muscle cells |
| abstract | The present invention relates to a kind of isolated culture method of chicken embryo arteria pulmonalis smooth muscle cells, the described method comprises the following steps：Separation chicken embryo herringbone pulmonary arterial vascular simultaneously ensures its moistening with PBS, prune away except connective tissue tiny around blood vessel, herringbone blood vessel is separated into after two blood vessels a rip cutting is carried out to two blood vessels respectively, the blood vessel after rip cutting is terminated into digestion with complete medium after 15 37 DEG C of 1 2min of digestion with trypsin solution and obtains postdigestive tissue block, postdigestive tissue block is cut into the tissue fritter that size is 0.4 0.6mm, tissue fritter is subjected to original cuiture in complete medium.Simple to operate, reproducible, the cell purity of culture is high, and growth cycle is short, and the 3rd day i.e. visible cell of culture climbs out of, and original cuiture can carry out Secondary Culture after 4 days, cell growth is vigorous. |
| priorityDate | 2015-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.