http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105021671-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-26 |
filingDate | 2015-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105021671-B |
titleOfInvention | A kind of cell pre-treating method of Electrochemical Detection |
abstract | A kind of cell pre-treating method of Electrochemical Detection, it is related to a kind of cell pre-treating method of Electrochemical Detection.The present invention is not complete enough in order to solve existing cell pretreatment technology release electroactive material, and so as to can not truly reflect the problem of cytoactive changes, the inventive method is:Cell to be detected is inoculated in culture dish, is placed in 37 DEG C, CO 2 Volumetric concentration is to be cultivated in 5% incubator, then removes culture medium, adds hypotonic lysis liquid, adjusts cell concentration, then under water bath condition, cracking, that is, is completed.The compound cracking of cell in-situ of the present invention, being completely severed for cell membrane is realized, electrochemical signals are greatly enhanced, and handle and simplify, not only strengthen electrochemical detection signal, it is often more important that Electrochemical Detection result more accurately reflects the truth of endocellular electricity activity substance content.The present invention is applied to field of bioanalysis. |
priorityDate | 2015-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 15.