http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105018647-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-708 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 2015-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105018647-B |
| titleOfInvention | A kind of kit and its detection method based on the accurate quantitative typing detection HPV16/18 of digital pcr |
| abstract | The present invention discloses a kind of kit and its detection method based on the accurate quantitative typing detection HPV16/18 of digital pcr；Kit includes DNA extracting solutions, digital pcr reaction buffer A, digital pcr reaction buffer B, HPV16 viral gene positive quality control, HPV18 viral genes positive quality control, negative Quality Control and inner mark solution；Method includes the following steps：1）By inspection sample treatment；2）The processing of quality-control product；3）Prepare digital pcr reaction mixture；4）Micro- reaction drop is generated, and carries out digital pcr reaction amplification；5）Fluorescence signal is read, HPV types is analyzed and calculates copy number；The present invention is easy to operate independent of external reference material and standard curve, directly to the accurate absolute quantitations of HPV parting at the same time；And high sensitivity, background dna and matrix interference can be reduced, quantitative very small amount HPV, as low as 1 copy of detection limit. |
| priorityDate | 2015-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 285.