| abstract |
The invention discloses a constant temperature index amplification technology based on triple amplification reaction connection in series and an application of the constant temperature index amplification technology in microRNA detection. According to the invention, a special neck ring structure is designed as a medium body, a constant temperature index amplification reaction is triggered, target sequence cycle is fromed under strand displacement reaction effect of polymerase, a lot of short chain DNA fragments are generated through amplification in short time, deoxyribozyme is activated, a fluorescence substrate is cut, target sequence cycle is formed, constant temperature index amplification reaction is carried out, series connection of a triple cycle amplification process of a deoxyribozyme catalytic reaction is carried out, then a rapid constant temperature index amplification technology with high efficiency is established, and the constant temperature index amplification technology can be used for microRNA ultra-sensitive fluorescence detection. |