http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105018552-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2015-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105018552-B |
| titleOfInvention | The preparation method of fluorescence protein is merged in a kind of Escherichia coli |
| abstract | The invention belongs to fluorescence proteins in biotechnology and recombinant antibodies field, particularly relate to merge the preparation method of fluorescence protein in a kind of Escherichia coli.Preparation method is into mosaic gene by AFP single-chain antibody genes and allophycocyanin α subunit genes by linker sequence assemblies, and co-express the mosaic gene, phycobniliprotein lyase genes and phycobilin biosynthetic enzyme genes in Escherichia coli, obtain the fusion fluorescence protein of covalent bond phycoerythrobilin or the fusion fluorescin of covalent bond phycocyanobilin.The present invention is that single-chain antibody and allophycocyanin α subunit fusion proteins are produced in Escherichia coli body using biological technique method, is a kind of environmental-friendly, at low cost preparation method.The fusion protein can be used for the fields such as biology and biomedical detection. |
| priorityDate | 2015-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 69.