http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105018511-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-22 |
| filingDate | 2015-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105018511-B |
| titleOfInvention | A kind of method of external enzyme reaction method for synthesizing phloroglucinol and application |
| abstract | The invention discloses a kind of method of external enzyme reaction method for synthesizing phloroglucinol and applications, belong to technical field of bioengineering.Method provided by the present invention is that structure contains acetyl-CoA-synthetase ACS genes respectively, acetyl-CoA carboxylase subunit gene accA, acetyl-CoA carboxylase subunit gene accB, acetyl-CoA carboxylase subunit gene accC, the recombinant vector of acetyl-CoA carboxylase subunit gene accD and polyketide synthases gene phlD, overexpression after recombinant vector being transformed into respectively in host strain, utilize ultrasonic disruption thalline, enzyme solution is obtained after isolating and purifying, and passes through external enzyme reaction method for synthesizing phloroglucinol after mixed enzyme solution and reaction substrate in proportion.The present invention is realized through external enzyme reaction method for synthesizing phloroglucinol, and can be with each enzyme of accuracy controlling and the dosage of confactor. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109456927-A |
| priorityDate | 2015-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.