http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105018482-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-08 |
| filingDate | 2015-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105018482-B |
| titleOfInvention | A kind of heterozygosis tRNA and its application in glutathione peroxidase is prepared |
| abstract | A kind of heterozygosis tRNA and its application in glutathione peroxidase is prepared, belong to biological technical field, are related to sequence SEQ ID No:1~16, it is by 90 base compositions, the substrate tRNA that can either be synthesized as selenocysteine, can be recognized again by the elongation factors EF Tu of Escherichia coli itself.First heterozygosis tRNA genes are obtained with gene synthesis, synthesize or expand again GPX genes, both are assembled on the same secreting type prokaryotic expression carrier that can express heterozygosis tRNA, convert TAG engineering bacterias, the induced expression in the presence of sodium selenite, GPX catalytic group Sec is incorporated into the substrate-binding site of albumen by way of conventional amino acid enters peptide chain, its high GPX activity is assigned.The inventive method is simple, and zymoprotein vigor and yield height, stability are good, so as to solve natural GPX limited sources and property instability problem. |
| priorityDate | 2015-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 319.