http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105018422-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
filingDate | 2015-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105018422-B |
titleOfInvention | A kind of method of human umbilical cord mesenchymal stem cells prepare with scale |
abstract | The invention discloses a kind of methods of human umbilical cord mesenchymal stem cells prepare with scale.The present invention includes the following steps:Collect umbilical cord and placenta;Umbilical cord separation and Extraction hUC MSC, early period is taken to contain fetal calf serum using tradition(Fetal bovine serum, FBS)DMEM/F12 culture solutions carry out amplification cultivation;Later stage is with umbilical cord with source placenta homogenate(Placental homogenate extraction, PHE) it is further cultured for for hUC MSC instead of FBS, to obtain hUC MSC raw cells.The present invention is under the premise of being not used commercialization serum free medium, you can realizes the external prepare with scale of hUC MSC, while solving cow's serum residue problem again, and saves great amount of cost. |
priorityDate | 2015-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 121.