http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105010399-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01P3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N63-00 |
| filingDate | 2014-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105010399-B |
| titleOfInvention | The isolation and purification method of antifungus active substance in a kind of streptomyces lydicus zymotic fluid |
| abstract | The invention discloses a kind of isolation and purification method of antifungus active substance in streptomyces lydicus zymotic fluid, step is：（1）The zymotic fluid that streptomyces lydicus S.lydicus E9 preserving numbers are CGMCC NO.3075 is centrifuged, collects supernatant, adds flocculant, mixing stands, filters to obtain filtrate；（2）Filtrate rinses depigmentation and unadsorbed impurity through macroreticular resin chromatographic column, with methanol aqueous solution, then with the activity eluted ingredient of ethanol water；（3）Merge active eluant and be concentrated to give concentrate；（4）Concentrate carries out sieve chromatography through sephadex chromatography column, is eluted with ethanol water, is in charge of collection eluent, and by collection sequence number consecutively, detect its antifungal activity respectively, collect antifungal activity component.It is simple and quick, easy to operate, at low cost that the present invention isolates and purifies flow.Three waste discharge is few.Purification process is mild, and the purity of active material is higher.Eluant, eluent toxicity is low, and recovery processing is simple. |
| priorityDate | 2014-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.