http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105002567-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2015-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105002567-B |
| titleOfInvention | Simplify the construction method for the sequencing library that methylates without reference gene group high flux |
| abstract | The invention provides a kind of construction method for simplifying the sequencing library that methylates without reference gene group high flux, to the digestion of sample gene group and plus A, obtaining 5 ' ends has phosphorylation modification and 3 ' cohesive end A DNA fragmentation, and it is connected from the joint that methylates containing different bar code sequencesï¼›Connection product is divided into two groups, and one group is entered performing PCR amplification, another group converted through bisulfite after expand again, obtain two groups and contain same bar code, only without the different amplified production sequence in the cytimidine site methylatedï¼›Two groups of PCR primers are mixed respectively, and selection identical length-specific fragment sequence carries out low circulation PCR, and amplified production isolates and purifies structure high flux and simplifies the sequencing library that methylates respectively.This method flux is high, cost is low, sequence is consistent, without reference to genome, is had a extensive future in the DNA methylation epigenetics research field for largely assembling infull species without reference gene group or reference gene group. |
| priorityDate | 2015-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 18.