http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105002240-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-315 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04 |
| filingDate | 2015-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105002240-B |
| titleOfInvention | A kind of antibiotic and the preparation method and application thereof |
| abstract | The present invention relates to a kind of antibiotic and the preparation method and application thereof, preparation method is：It first will be after 11454 actication of culture of Lactococcus lactis ATCC, picking single bacterium colony is cultivated, and centrifugation removal strain obtains zymotic fluid, and rotary evaporation concentration is recycled after zymotic fluid pH is adjusted to 2, pH is adjusted to again to take supernatant after initial value is then centrifuged for, Fermented Condensed liquid is obtained after filtration treatment；Fermented Condensed liquid is mixed with PBS buffer solution, adds Triton X 114 and MgSO 4 The double-aqueous phase system of composition, centrifugation make aqueous two-phase reach balance each other after take phase, then the antibiotic containing nisin and Triton X 114 is made after being diluted with PBS buffer solution.The antibiotic of the present invention has significant fungistatic effect to gram-positive bacterium.The antibiotic of the present invention includes Nisin and Triton X 114, and the combination of the two makes antibiotic have higher potency.The extraction process of the present invention is simple, at low cost, is suitable for the antibiotic of intermittence and large-scale production processing high-titer, high yield. |
| priorityDate | 2015-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.