http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105002227-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-6427 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-64 |
filingDate | 2015-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105002227-B |
titleOfInvention | A method of shake flask fermentation schizochytrium limacinum is improved by feed-batch process and produces docosahexaenoic acid |
abstract | The method that shake flask fermentation schizochytrium limacinum produces docosahexaenoic acid is improved by feed-batch process the invention discloses a kind of, belongs to field of microbial fermentation.The present invention uses the culture medium containing trace element for fermentation medium, in fermentation process stream plus glucose solution maintain zymotic fluid in glucose content between 30~50g/L, while flow plus one time fermentation liquid product 1/5~1/4 210.0~320.0g/L mixed nitrogen solution.The present invention can ensure demand of the middle and later periods schizochytrium limacinum to carbon source of fermenting by stream plus glucose, improve single batch ferment strength in shaking flask;By stream plus mixed nitrogen can improve schizochytrium limacinum the shaking flask middle and later periods fermentation level;The accumulation that aforesaid operations can largely improve the accumulation of schizochytrium limacinum biomass during shake flask fermentation, promote DHA in mycelium.The present invention has great meaning to the pilot scale culture production DHA of schizochytrium limacinum. |
priorityDate | 2015-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.