http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105002195-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 |
| filingDate | 2015-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105002195-B |
| titleOfInvention | A kind of pig blue-ear disease recombinant vaccine |
| abstract | The invention discloses a kind of pig blue-ear disease recombinant vaccines, and PRRS virus ORF3 genes and ORF5 gene tandems are constructed fusion ORF3‑ORF5 , it is inserted into the safely expressed carrier pCB130NG of the double T DNA of fungi specificity, obtains a kind of expression vector pCB130NG ORF3‑ORF5 Edible and medicinal fungi protoplast is converted using PEG methods, agrobacterium-mediated transformation or electric shocking method, it is identified as positive mycelium, culture obtains T1 for fructification, it is filtered out containing only target gene and the individual of the fructification without selectable marker gene using PCR, culture obtains the transgenosis edible and medicinal fungi for stablizing expression PRRS virus gene to T2 generations.By the fermented and cultured of transgenic fungus mycelia, mycelia is collected by centrifugation, after drying or freeze-drying, crushing prepares pig blue-ear disease recombinant vaccine.Using between pig blue-ear disease vaccine each batch produced by the invention, quality difference is small, yield is big, at low cost, and can have extensive Social benefit and economic benefit directly by oral Gavage animal. |
| priorityDate | 2015-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 395.