http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105002147-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-03004 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
| filingDate | 2015-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105002147-B |
| titleOfInvention | The mutation glucose oxidase and its encoding gene and application that expression quantity improves |
| abstract | The present invention relates to genetic engineering fields, and in particular to the mutation glucose oxidase and its encoding gene and application that expression quantity improves, amino acid sequence is as shown in SEQ ID NO.1.The present invention uses site-directed mutagenesis technique by glucose oxidase (GOD) gene (Genebank of aspergillus niger Aspergillus niger GIM 3.452 (CICC 2377):FJ979866.1 rite-directed mutagenesis) is carried out, it is Y76C and Q279K to make its Amino acid sequence mutants site；Mutator clone is connected to yeast expression vector pPICZ α A, converts Pichia pastoris X33, screening obtains the glucose oxidase Pichi strain P.pastoris X33 pPICZ α A GODmut of expression quantity raising；Zymologic property measure show the glucose oxidase gene of mutation can stablize in Pichia pastoris, efficient expression and hereditary, the enzymatic activity and stability of the glucose oxidase expressed by mutant strain are significantly higher than starting strain.This haves laid a good foundation for the large-scale production of glucose oxidase. |
| priorityDate | 2015-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.