http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105002142-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P3-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2015-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105002142-B |
| titleOfInvention | Directly reprogram the method and application that mouse liver cell is beta Cell of islet |
| abstract | The invention discloses methods and application that directly reprogramming mouse liver cell is beta Cell of islet.Make liver cell reprogramming be the method for beta Cell of islet the present invention provides a kind of, includes the following steps: Pdx1 protein coding gene, Pax4 protein coding gene and Neurod1 protein coding gene transfecting in vitro liver cell, obtain beta Cell of islet；It is an advantage of the present invention that first, using non-viral EntransterTM-D transfection reagent, not only transfection efficiency with higher, while substantially increasing the safety transfected during directly reprogramming.Second, according to the report of document, filters out to be suitable for directly reprogramming and combine Pdx1, Pax4, Neurod1 for the new best transfection of beta Cell of islet.Third filters out the best transfection period for 24 hours according to the sequencing that each transcription factor plays a role, to improve the maturity problem that directly reprogramming is islet cells, reprogramming efficiency is up to 23% in this research. |
| priorityDate | 2015-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 193.