http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104991019-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 |
| filingDate | 2015-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104991019-B |
| titleOfInvention | Gelsemine and the liquid chromatography-tandem mass of koumine in biological material |
| abstract | The present invention relates to the Liquid Chromatography-Tandem Mass Spectrometry detection method of gelsemine and koumine in biological material, comprise the steps: to take biological fluid or biological tissue samples shreds in tool plug test tube；Above-mentioned sample adds a certain amount of sodium hydroxide solution and adjusts pH9～10, adds ethyl acetate, and vibrate 10min, high speed centrifugation 10min；After separating organic facies, add organic solvent and carry out extracting for the second time, merge twice organic facies, be placed on 50 DEG C of concentrating instruments and volatilize；Residue initial flow phase constant volume, crosses the 0.22 organic filter membrane of μm micropore, and filtrate is for liquid chromatography tandem spectrometer analysis.The detection method of the present invention is simply efficient, rapid sensitive, accuracy are high, have wide applicability, it is possible to is applied to the qualitative, quantitative inspection of gelsemine and koumine in biological sample, and is applicable to vitro samples and the inspection of suspicious material evidence. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109655563-A |
| priorityDate | 2015-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.