http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104988177-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 |
| filingDate | 2015-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104988177-B |
| titleOfInvention | A kind of preparation method of transgenic watermelon plant |
| abstract | The present invention provides a kind of preparation method of transgenic watermelon plant, belong to genetically modified plants preparation field.This method is to infect watermelon ripe cotyledon explant using the Agrobacterium for being transferred to foreign gene；It is transferred to co-culture afterwards and is cultivated on base, the co-cultivation base co-cultures base by 3 layers of aseptic filter paper and suitable liquid and forms；Explant is transferred to screening and culturing medium afterwards, early period uses the selective agent of low concentration, and the later stage gradually steps up screening agent concentration, screen, take root by bud elongation, hardening, transplanting, obtain being transferred to the watermelon plant of foreign gene, Molecular Identification the results show foreign gene is successfully transferred to and can stablize expression.The method of the present invention improves explant cell viability, transformed cells number is more, transformed cells are good with regenerative cell's plyability, and escape body frequency is low, high conversion rate, transfer-gen plant robust growth, take root, domestication survival rate is high, is converted suitable for different foreign genes and agrobacterium strains type, and repeatability is good, has good economic value. |
| priorityDate | 2015-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.