http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104987433-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08B37-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P39-06 |
| filingDate | 2015-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104987433-B |
| titleOfInvention | Preparation method of RG-I type lycium barbarum pectin with anti-aging activity |
| abstract | The invention relates to a preparation method of RG-I type lycium barbarum pectin with anti-aging activity. The method comprises the following steps: (1) water is added into lycium barbarum, and the mixture is subjected to microwave-ultrasonic assisted extraction, filtering and concentration, such that a concentrate liquid is obtained; (2) anhydrous ethanol is added into the concentrate liquid, and the materials are well mixed; the mixture is allowed to stand and is subjected to centrifugation, such that a precipitation product is obtained; (3) the precipitation product is re-dissolved, and the solution is subjected to centrifugation and drying, such that lycium barbarum polysaccharide is obtained; (4) lycium barbarum polysaccharide subjected to DEAE-cellulose column chromatography separation, and is eluted; and dialysis desalination and lyophilization are carried out, such that lycium barbarum neutral polysaccharide LBP-N and lycium barbarum acidic polysaccharide LBP-A are respectively obtained; (5) lycium barbarum acidic polysaccharide LBP-A is subjected to Sepharose CL-6B gel filtration column chromatography separation, and is eluted; dialysis desalination and lyophilization are carried out, such that lycium barbarum acidic polysaccharides LBP-A-1 and LBP-A-2 are obtained; (6) lycium barbarum acidic polysaccharide LBP-A-1 is subjected to DEAE-Sepharpse Fast Flow anion exchange column chromatography purification; continuous gradient elution is carried out with a 0-0.5 mol/L NaCl aqueous solution; and dialysis desalination and lyophilization are carried out, such that the anti-aging RG-I type lycium barbarum pectin pure product is obtained. The method provided by the invention has the advantages of simple operation and high extraction rate |
| priorityDate | 2015-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 125.