http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104977200-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N24-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
| filingDate | 2015-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104977200-B |
| titleOfInvention | A kind of pig stomach intestinal contents metabolism group extracting method |
| abstract | The invention discloses a kind of pig stomach intestinal contents metabolism group extracting method, method are as follows：Weigh the gastrointestinal content 1g to thaw, pH value is adjusted to 8.0 using 1M NaOH solutions and 1M HCl solutions, then the content of 0.1g mixings is weighed, add 0.1M phosphate buffers (pH value 8.0) 1mL, after liquid nitrogen flash freezer, in water-bath ultrasound and thaw, be placed in (20Hz in historrhexis's instrument, 30s) crush, 3 such multigelation, ultrasound and break process circulations.Finally, 4 DEG C, 5 000rpm centrifugation 10min, supernatant 0.6mL is taken to add 5mm nuclear magnetic tubes standby.Detectable 51 kinds of metabolin, wherein, 20 kinds of amino acid, 7 kinds of short chain fatty acids, 5 kinds of tricarboxylic acid cycle metabolin, 19 kinds of other metabolins, whole extraction process only needs 3h.Spectral peak drift is influenceed very little by pH value.Therefore, this method not only increases the recovery rate of metabolin, improves the quality of nuclear magnetic spectrogram, and simplifies extraction flow. |
| priorityDate | 2015-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.