http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104975044-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 |
| filingDate | 2015-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104975044-B |
| titleOfInvention | It is a kind of target SRSF6 slow virus interference carrier building and its application in treatment of colorectal cancer |
| abstract | The invention discloses the shRNA, Lentiviral and its construction methods that inhibit SRSF6 gene expression, the shRNA sequence is F:5'-CCGGCGTACAGAATACAGGC TTATTCTCGAGAATAAGCCTGTATTCTGTACGTTTTTG-3', R:5'-AATTCAAAAACGTACAGAATACAGGCTTAT TCTCGAGAATAAGCCTGTATTCTGTACG-3'；The Lentiviral is to construct PLKO.1-SRSF6-shRNA in above-mentioned shRNA insertion vector plasmid PLKO.1；Transfection HEK293T cell in itself and packaging plasmid PSPAX2 and PMD2.G is obtained into slow virus suspension after carrier sequence verification is correct；Infection colorectal cancer cell is removed with this suspension, is then packed and whether is succeeded with puromycin screening verification virus；Western Blot verifies its expression that can be substantially reduced SRSF6；And it can significantly reduce the proliferative capacity and migration invasive ability of colorectal cancer cell；Wherein SRSF6 still counts for the first time the research of the effect of tumor cell migration invasive ability. |
| priorityDate | 2015-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.