http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104965017-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 |
| filingDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104965017-B |
| titleOfInvention | The assay method of hirudin anticoagulant hemase activity |
| abstract | The invention discloses a kind of assay method of hirudin anticoagulant hemase activity, by after hirudin of the known certain mass containing unknown activity and enough blood coagulation enzyme reaction, in crosslinking agent B S 3 Cross-linking reaction is carried out under effect, obtain containing hirudin after terminating crosslinking, first mixed system of fibrin ferment and hirudin blood coagulation enzyme compound, densitometric scan is used after electrophoresis, protein band density according to hirudin blood coagulation enzyme compound on the basis of the tester of known protein band density obtains the quality of hirudin blood coagulation enzyme compound, combined as 1: 5 with mass ratio according to hirudin and fibrin ferment and obtained the quality that hirudin blood coagulation enzyme compound draws the effective fibrin ferment for participating in reaction, the hirudin for drawing the certain mass further according to effective mass fraction of fibrin ferment and the Mass Calculation of hirudin in fibrin ferment suppresses the activity value of the activity value as hirudin anticoagulant hemase of fibrin ferment.The method of the present invention is simple to operate, accuracy is good, input and equipment requirement are low. |
| priorityDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.