http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104962511-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2015-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104962511-B |
| titleOfInvention | A kind of method of Muskrat fragrant secretory cell Isolation and culture and application |
| abstract | The invention discloses a kind of method of Muskrat fragrant secretory cell Isolation and culture and applications, belong to technical field of cell biology.The present invention is the scent gland tissue in the muskrat for secreting the fragrant phase taken, it is immediately placed in the phosphate buffer solution containing penicillin and streptomysin of 4 DEG C of precoolings and starts the cleaning processing, remove the peripheral organization of scent gland, scent gland tissue after cutting process and the phosphate buffer solution containing penicillin and streptomysin being pre-chilled using 4 DEG C are centrifuged after being cleaned, Type Ⅱ collagen enzyme is recycled to carry out digestion process, it is centrifuged after digestion, the precipitate that culture of isolated obtains.This invention simplifies the Isolation and culture step of Muskrat fragrant secretory cell, uniform fritter scent gland tissue only need to be obtained, directly can be carried out culture operation, and can guarantee the growth conditions of cell close to internal microenvironment.Meanwhile the present invention can be simple and quick obtain the higher secretory cell of purity, greatly shorten the process that secretory cell isolates and purifies. |
| priorityDate | 2015-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.