http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104946716-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cc30f385f064e1e202529d37f1ec892a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K8-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61Q19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 |
| filingDate | 2015-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1ccde29f442ae2f41e1219124f766d9 |
| publicationDate | 2015-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104946716-A |
| titleOfInvention | Sericin protein polypeptide 1 as well as preparation and application thereof |
| abstract | The invention discloses a sericin protein polypeptide, and relates to the field of polypeptide preparation in biotechnology. The sequence of the sericin protein polypeptide is MSPSRATTTVHHTDNASIRGP. The sericin protein polypeptide is prepared according to the following steps: (1) taking 500 g of sericin powder to be added into deionized water to be dissolved according to the mass/volume ratio of the 1:(1-3); (2) quick-cooling a sericin solution to be 4-10 DEG C; (3) centrifuging at 4-10 DEG C and at 10000-15000 rpm for 30-60 min, and taking a supernatant; (4) adopting a rotary evaporimeter to concentrate the supernatant; (5) after the solution is cooled, adding hydrolase, and reacting at 20-40 DEG C for 30-60 min; (6) centrifuging at 2000-3000 rpm for 5-10 min and taking a supernatant; (7) adding the supernatant into a buffer solution with the pH of 5.0-6.0, stirring, and centrifuging at 10000-15000 rpm for 30-60 min to obtain sediment; (8) redissolving the sediment into a buffer solution with the pH of 6.4-7.2, and adopting a dialysis method to separate the polypeptide of which the molecular weight is 1600-3400 Da. The sericin protein polypeptide is applied to cosmetic preparation; the purity of the prepared sericin protein polypeptide is greater than 70%; experiments show that the polypeptide has the function of moisture preservation, and is equivalent to the original sericin protein in function. |
| priorityDate | 2015-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.