http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104928350-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 |
| filingDate | 2015-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104928350-B |
| titleOfInvention | Nine kinds of external depression effect rapid screening methods of people's liver CYP450 enzymes |
| abstract | The invention discloses the rapid screening method of 14 kinds of probe substrates of application and 16 kinds of probe reaction comprehensive assessments, 9 kinds of external depression effects of people's liver CYP450 metabolic enzymes.It is quick comprehensively to assess depression effect of the test-compound to metabolic enzyme the invention mainly relates to the metabolic activity change that method and LC/MS/MS combination 9 kinds of people's liver CYP450 enzymes of monitoring are incubated using external mixed probe.This method has considered the selectivity and diversity of probe substrate, influencing each other between probe substrate, temperature incubates different incubation conditions (organic solvents in system, buffer solution, BSA addition etc.) to the activity influence of probe reaction, and every factor such as the enzyme kinetics feature of 16 kinds of probe reactions under selected incubation conditions, and combine high sensitivity, the LC MS/MS technologies of high selectivity, establish a kind of brand-new evaluating in vitro system, to more accurate can more fully predict test-compound to 9 kinds of issuable depression effects of people's liver main metabolic enzyme in the high flux screening of new drug development, improve the foresight of later stage metabolic interaction. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022024056-A1 |
| priorityDate | 2015-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 138.