http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104928270-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 |
| filingDate | 2014-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104928270-B |
| titleOfInvention | The recombinant basic zytase and its construction method of one group of ethylenediamine tetra-acetic acid of resistance to chelating agent |
| abstract | The invention discloses the alkalescent xylanase and encoding gene and its construction method of one group of resistance to EDTA.Alkalescent xylanase enzyme coding gene xynA is mutated using side-directed mutagenesis, obtain mutator xynA M W19Y, xynA M W19F and xynA M W19H, it is induced in E.coli BL21 (DE3), expresses, purify, enzyme after purification is subjected to enzyme activity determination, obtained xylanase mutant XynA M W19Y, XynA M W19F and XynA M W19H has preferable resistance to EDTA abilities, wherein in pH9.2,60 DEG C, under conditions of 5mM EDTA, the enzyme activity of mutant W20Y is higher than wild type XynA by nearly 20%.This haves laid a good foundation for the extensive use of alkalescent xylanase. |
| priorityDate | 2014-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.