http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104928224-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2015-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104928224-B |
| titleOfInvention | A kind of ferulic acid production engineering bacterial strain, construction method and bioconversion method |
| abstract | The invention discloses a kind of ferulic acid production engineering bacterial strain, construction method and bioconversion methods, the engineered strain of offer incorporates new enzyme and couples system, so that chinese cymbidium alcohol oxidase gene, coniferyl-alcohol dehydrogenase gene and pine and cypress aldehyde dehydrogenase gene is co-expressed in Escherichia coli using strong promoter, ferulic acid is generated using enzymatic reaction catalysis eugenol.The system couples Xylose reductase simultaneously, xylose is restored in process of production, regenerating oxidation type coenzyme nicotinamide adenine dinucleotide and nicotinamide-adenine dinucleotide phosphate simultaneously maintain the coenzyme balance of ferulic acid bioconversion systems, to improve the conversion ratio and yield of product ferulic acid.Compared with currently available technology, the present invention provides effective, simple implementation to the safe biologic conversion of medical intermediate product ferulic acid, and conversion ratio reaches 95% or more, and final product asafoetide acid concentration reaches 11g/L, has higher industrialization value. |
| priorityDate | 2015-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 364.