http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104911262-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate | 2015-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104911262-B |
titleOfInvention | A kind of dalbergia odorifera tar spot bacterium molecule detection primer and quick determination method |
abstract | The invention belongs to the Molecular Detection field of plant disease, and in particular to a kind of molecular detection primer and its detection method of dalbergia odorifera black mole germ.Pass through nested PCR amplification, PCR primer Amplification Analysis, agarose gel electrophoresis and result judgement using a pair of the dalbergia odorifera black mole germ special primers designed.If yellow wingceltis black mole bacterium in sample be present, 273bp purpose band occurs in Ago-Gel under ultraviolet light, if not occurring band, yellow wingceltis black mole bacterium is not present in representative.The primer specificity of the present invention is strong, can distinguish yellow wingceltis black mole bacterium, dogstail black mole bacterium, firm bamboo black mole bacterium and be isolated from dalbergia odorifera, other pathogens of Burma padauk and endogenetic fungus;The primer high sensitivity, lower limit is can detect to 100ag/ μ L yellow wingceltis black mole bacterium genomic DNA;It is of the invention quick, accurate testing result is can obtain in 8 hours, and at least need 4 days or more than 1 week using traditional disease screening method. |
priorityDate | 2015-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.