http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104911202-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P29-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 |
| filingDate | 2015-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104911202-B |
| titleOfInvention | A kind of preparation method of analgesic activities polypeptide |
| abstract | The invention discloses a kind of preparation method for having analgesic activities polypeptide, belong to genetic engineering field；The preparation method is as follows：Pokeweed antibiotic peptides PaAMP1 gene orders are optimized according to e. coli codon frequency of use, prokaryotic expression carrier pET 32a carriers are connected into after chemical synthesis, recombinant plasmid is then imported into Escherichia coli Origami（DE3）Bacterial strain, after IPTG is induced, realizes the solution expression with high efficiency of restructuring PaAMP1；Restructuring PaAMP1 is isolated and purified using nickel ion affinity chromatograph method；The present invention confirms that restructuring PaAMP1 has significant analgesic activities first by the experiment of mouse acetic acid twisting, has potential application value in analgesia field；Since PaAMP1 derives from plant dyers' grapes, which is easy to large-scale plantation, therefore its resource, is expected to the industrialization from the dyers' grapes of large-scale plantation and extracts natural analgesic polypeptide, meet medicament research and development and clinical demand more than marine conidae aboundresources. |
| priorityDate | 2015-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.