http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104911175-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-14 |
filingDate | 2015-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104911175-B |
titleOfInvention | Aminosilylation magnetic nano-particle co-immobilization laccase and mediator system and preparation method thereof |
abstract | The invention discloses a kind of Aminosilylation magnetic nano-particle co-immobilization laccase and mediator system and preparation method thereof, which is that laccase and mediator are immobilized in jointly on Aminosilylation magnetic nano-particle.In Fe 2+ And Fe 3+ NaOH solution is added in solution and carries out the obtained Fe of coprecipitation reaction 3 O 4 Nano-particle;Using 3 aminopropyl triethoxysilanes(APTES)The magnetic nano-particle that modification obtains surface amination is carried out to it;Using glutaraldehyde as crosslinking agent, the part amino on magnetic nano-particle surface is grafted 4 NH 2 TEMPO has been grafted the nano-particle of mediator TEMPO;It is finally added in laccase solution, is obtained through glutaraldehyde cross-linking immobilization laccase.Laccase and mediator are fixed on magnetic carrier by the present invention altogether for the first time, and enzyme easily can be recycled and be reused with mediator by external magnetic field, to improve the utilization rate of enzyme, reduce cost. |
priorityDate | 2015-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 84.