http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104911144-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 |
| filingDate | 2015-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104911144-B |
| titleOfInvention | The isolated culture method of one breeder germinal crescent source PGCs |
| abstract | The isolated culture method of breeder germinal crescent source PGCs of the present invention, includes the following steps：(1) separation method is that the clean chicken embryo polluted without yolk is taken out from hatching egg by pasting filter membrane method, and germinal crescent tissue is then obtained from chicken embryo；(2) with pancreatin by germinal crescent tissue digestion at unicellular, then the PGCs in unicellular is purified；(3) PGCs after purification is placed in the cultivating system containing BRL 3A cell feeder layers and PGCs complete culture solutions and is cultivated.PGCs in the present invention derives from chicken embryo germinal crescent, the PGCs contents of 5 8HH phase chicken embryos germinal crescents are higher, with more preferable proliferation activity and maintain high-caliber versatility, it pastes filter membrane method and detaches chicken embryo blastodisc, it is easy to operate, it is reproducible, the clean chicken embryo of no yolk pollution is isolated from hatching egg, obtains more pure PGCs.The PGCs being separately cultured can be largely proliferated, and be operated for subsequent experimental. |
| priorityDate | 2015-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 132.