http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104897902-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4fcbe99de2e1bc092edc299b68dac02b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531 |
| filingDate | 2015-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb8a77e24a001fd850cd722fbd5edb11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47a0f9b76128138060877305b98c7849 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96dacce4b6738797bdb54d08813cf732 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_181419b0a3beebfdedccbbae1a454fb8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7dc7cdc7e9af20d0dd00e374552fdd73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d4ff7fc8080b4d0476c9eccc21cc2de |
| publicationDate | 2015-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104897902-A |
| titleOfInvention | Method for detecting soybean antigen beta-conglycinin |
| abstract | The invention discloses a method for detecting soybean antigen beta-conglycinin. A direct ELISA method for detecting soybean antigen beta-conglycinin is established. The envelope antigen is determined to be 1mu g/mL by virtue of a square matrix experiment, the optimal dilution degree of the antibody is 1:400, the optimal confinement time is determined to be 58.31 minutes by virtue of the orthogonal response surface experiment design, the optimal reaction time of an enzyme-labeled antibody is 139.16 minutes, the optimal reaction time of substrate solution action is 19.43 minutes, the sensitivity is 1ug/ml, and the linear detection range is between 20ng/ml and 640ng/ml. The mean number of the variation coefficient (CV%) between the plates is 8.16 percent, the mean number of the variation coefficient (CV%) in the plates is 6.91 percent, and the cross reaction is smaller than 20 percent. The detection method disclosed by the invention has the characteristics of relatively simple sample pretreatment, rapidness, low cost, low instrumentation degree, short time consumption and the like, can be applied for detecting the content of glycinin in soybean and deep processing products and is applied to field monitoring and large-scale sample screening. |
| priorityDate | 2015-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.