http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104894219-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-10 |
| filingDate | 2015-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104894219-B |
| titleOfInvention | Method that is a kind of while detecting Salmonella in Food and staphylococcus aureus |
| abstract | Method that is a kind of while detecting Salmonella in Food and staphylococcus aureus, comprises the following steps：1）Food samples to be measured are diluted with reference to GB 4789, obtain 1:The 10 even liquid of sample；50 65 wt% aminotriacetic acid solution are added into the even liquid of sample, the even liquid of sample is 57 with aminotriacetic acid liquor capacity ratio:1, vibration, layering is centrifuged after adding phosphate buffer or phosphate buffer containing 0.05% polysorbas20, abandons upper strata；2）To through step 1）After the mixing of sample addition immunomagnetic beads, vibration after processing, in stratification on magnetic frame, upper strata is abandoned, lower floor's sample is washed；Immunomagnetic beads is made using the salmonella and Staphylococcus aureus antibody of Streptavidin modification magnetic bead couple biotin mark；3）Phosphate buffer is added to the sample after washing, vibration, is applied to LB flat boards, culture observation.Detection method is easy to operate, precise and high efficiency, cost are low, two kinds of object bacterias of Sync enrichment, detection time can be foreshortened into 25h by 72h. |
| priorityDate | 2015-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.