http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104894091-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01139 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96 |
| filingDate | 2015-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104894091-B |
| titleOfInvention | A kind of glycosyl modified method for improving enzyme heat stability of engineer |
| abstract | The present invention provides a kind of glycosyl modified methods for improving enzyme heat stability of engineer, belong to bioengineering field.The present invention to zymoprotein primary sequence and space structure by being combined analysis, N glycosylation sites are designed on zymoprotein subunit faying face, or N glycosylation sites are designed inside its subunit on die body faying face, glycosylation modified feature recognition enhancing sequence of N are introduced using rite-directed mutagenesis, so that sugar chain is formed specific glycosyl hairpin structure between zymoprotein subunit or forms specific glycosyl gasket construction between die body inside zymoprotein subunit, the rigidity for increasing zymoprotein tertiary structure to greatest extent stablizes stress of its space conformation from hot environment.The zymoprotein remodeling method of the present invention, is greatly improved the thermal stability of zymoprotein, and the method achieve the thermal stability of the raising zymoprotein of human controllable, and improve enzyme activity to a certain extent, enhance its catalysis characteristics. |
| priorityDate | 2015-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 185.