http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104894048-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2015-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104894048-B |
| titleOfInvention | A kind of method for improving Clostridium beijerinckii forulic acid resistance |
| abstract | The invention provides a kind of method for improving Clostridium beijerinckii forulic acid resistance, belong to gene engineering technology field.This method is to inactivate FMN reductase gene of the coding in Clostridium beijerinckii dependent on NADPH, the gene is set to be unable to normal expression in Clostridium beijerinckii, described Clostridium beijerinckii is Clostridium beijerinckii NCIMB 8052, described coding is Cbei_4693, its nucleotide sequence such as SEQ ID NO dependent on NADPH FMN reductase genes：Shown in 1.The present invention makes coding in Clostridium beijerinckii be inactivated dependent on NADPH FMN reductase genes by two type introne gene Knockouts.The recombinant bacterial strain has preferable mitotic stability, when the concentration of forulic acid in culture medium is 0.5g/L, the butanol yield of starting strain 8052 is 0.7g/L, and present invention structure recombinant bacterium butanol yield reaches 5.6g/L, illustrates to improve resistance of the Clostridium beijerinckii to forulic acid by the method for the present invention. |
| priorityDate | 2015-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 112.