http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104894047-B
Outgoing Links
Predicate | Object |
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classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-61 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate | 2015-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-104894047-B |
titleOfInvention | The construction method of the recombined bacillus subtilis of the epimerase of expression D psicoses 3 based on D alanine deficiency selection markers |
abstract | The construction method of the recombined bacillus subtilis of the epimerase of expression D psicoses 3 based on D alanine deficiency selection markers, belongs to enzyme gene field of engineering technology.Using bacillus subtilis 1A751 as starting strain, knock out D alanine racemases enzyme gene on its chromosome ( dal ) obtain D alanine deficiencies 1A751 ( dal ‑ );Clostruidium ATCC 35704 DPE enzyme genes are made into parent, P43 promoters is merged at its upstream and is built into P43 DPE, built to plasmid pUB110 (NCBI Gene ID:9507338) pUB P43 DPE are obtained in, the antibiotics resistance gene Kan and Blm on pUB P43 DPE are used dal Substitute, be built into pUB P43 DPE dal;Be transformed into again 1A751 ( dal ‑ ) in, recombined bacillus subtilis 1A751 pUB P43 DPE dal are obtained, are named as bacillus subtilis( Bacillus subtilis )SK38.001, deposit number CCTCC NO:M 2015257.Its zymotic fluid total enzyme activity reaches 16U/mL, has important industrial application value. |
priorityDate | 2015-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 432.