Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1c89ce4d9ac03f9acd8ddede1162f94d |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-20 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-775 |
filingDate |
2013-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ed5991607216afe919d9adad85b13021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19906ca36e91bd709bdd1c2b15840331 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c67dc106a949b76d5177c24d1a14c160 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3be2ea33868061eeb117e43c3ac18cb2 |
publicationDate |
2015-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-104854131-A |
titleOfInvention |
On-column refolding and purifying of lipoproteins |
abstract |
Provided are methods of refolding and purifying lipoproteins on a chromatography column. In particular methods of refolding ApoL1 polypeptides using a hydrophobic interaction column (HIC). The present invention provides methods for the purification of an active form of a human lipoprotein, such as ApoL1. More particularly, the present invention relates to a method for renaturing an inclusion body of proteins expressed in a large quantity in E. coli into an active form using a hydrophobic interaction column and removal of impurities, e.g., endotoxins. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111153985-A |
priorityDate |
2012-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |