http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104829715-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7c4e8f73abcdf5137584a53cfbdf4533 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 |
| filingDate | 2015-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_60d25fb996796df547d40db7f852ab5d |
| publicationDate | 2015-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104829715-A |
| titleOfInvention | Loach immunoglobulin preparation method |
| abstract | The invention provides a loach immunoglobulin preparation method, and belongs to the technical field of biology. The preparation method comprises the following steps: collecting fresh loach body surface mucus, adding 0.5-2 times of a phosphate buffer solution, filtering to remove impurities for standby use; slowly adding ammonium sulfate into the solution until the saturability reaches 15%-25%, standing at 3-5 DEG C for 12 hours, centrifuging for 10-20min to obtain a precipitate for standby use; dissolving the precipitate with the phosphate buffer solution in the above step, slowly adding ammonium sulfate until the saturability reaches 40%-55%, standing at 3-5 DEG C overnight, centrifuging for 10-20min to obtain a precipitate for standby use; dissolving the precipitate with the phosphate buffer solution in the above step, and desalting the solution; using an eluent for purification of an immunoglobulin solution in the elution rate of 0.2-0.5mL / min; and freezing and drying. The advantages and positive effects of the loach immunoglobulin preparation method are as follows: compared with the product in the prior art, the product has the characteristics of being high in extraction efficiency, highly active, low in cost, safe, green, pollution-free, simple in equipment, and the like. |
| priorityDate | 2015-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 117.