http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104829695-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 |
| filingDate | 2015-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104829695-B |
| titleOfInvention | A method of purifying alarelin |
| abstract | The invention discloses a kind of method purifying alarelin using mixed mode chromatographic separation material, mainly solve that existing purifying process yield is low, the higher technical problem of cost.Purifying alarelin includes the following steps：1）The acetonitrile solution or methanol that percent by volume is 5% are added in alarelin crude product, separation uses the chromatographic column that stationary phase is RPLC/IEX, wherein the trifluoroacetic acid aqueous solution of percent by volume 0.1% is A phases, the trifluoroacetic acid acetonitrile solution of percent by volume 0.1% is B phases, sample crude product solution is C phases, gradient elution purifying is carried out, the peptide solution of purpose peak value is collected；2) high-purity peptide solution after purification is turned into the exchange of salt method using HPLC and changes into acetate；3）Final high-purity peptide solution vacuum rotary steam concentrated frozen is dried, powdered finished product peptide is obtained.Present invention is mainly used for industrialized purification alarelins. |
| priorityDate | 2015-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.