http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104818292-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 |
| filingDate | 2015-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104818292-B |
| titleOfInvention | One kind is directed to sweet potato chlorotic stunt virus（SPCSV）RNA interference carrier and virus remove in application |
| abstract | Application the present invention relates to the interference vector for sweet potato chlorotic stunt virus gene RNase3 and its in virus removes.The construction method of the interference vector of sweet potato chlorotic stunt virus RNase3 genes, including designing two pairs of primers according to the nucleotide sequence of sweet potato chlorotic stunt virus RNase3 genes, using PCR method by RNase3 gene clonings to carrier T, using RNase3 T as template, enter performing PCR with two pairs of primers of design respectively to expand, the target gene RNase3 of acquisition is used respectively Kpn I and Xho I and Sac I and Xba After I digestions, the introne both sides of pBlue NiRIntron carriers are cloned into respectively in a manner of inverted repeat；Utilize Sac I and Kpn I double digestion pIntron RNase3IR carriers, reclaim the purpose fragment RNase3IR of the inverted repeats of gene containing RNase3, the purpose fragment are cloned on intermediate carrier pROK219.The present invention is directed to the SPCSV seriously occurred on sweet potato, successfully construct SPCSV RNAi carrier, and RNA is disturbed and is combined with stem tip tissue culture, virus-free techniques on sweet potato new system is established, effectively increases removing SPCSV efficiency and the yield of Virus-free Sweetpotato kind. |
| priorityDate | 2015-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.