http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104805112-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2015-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104805112-B |
| titleOfInvention | A kind of construction method of 3- hydracrylic acids Producing Strain recombinant plasmid |
| abstract | A kind of construction method of 3 hydracrylic acid Producing Strain recombinant plasmid belongs to biochemical industry production field.It is mostly relatively low in view of 3 hydracrylic acid engineering bacteria yield of existing production, it was found that suitable for klepsiella pneumoniae efficient tac promoters and the puuc aldehyde dehydrogenases that 3 hydroxy propanals are 3 hydracrylic acids can be converted, and recombination engineering is built with this.After the sequence of the enzyme coding gene is analyzed, chemical synthesis has been carried out to the gene.The promoter and enzyme are overexpressed in klepsiella pneumoniae after sequence verification, obtain recombination klepsiella pneumoniae pET (ptac puuc), measures it in vivo and in vitro to the catalytic capability of 3 hydroxy propanals.In vitro, the enzyme activity of 3 hydroxy propanal of enzyme pair reaches 26.31U/mg, is 6.9 times of primary klepsiella pneumoniae (3.82U/mg).Improved engineering bacteria is cultivated in 5L fermentation tanks, after medium component, the optimization of IPTG concentration conditions, 3 hydracrylic acid yield are up to 73g/L after 48h. |
| priorityDate | 2015-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 273.