http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104805098-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A62D101-26 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A62D3-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
| filingDate | 2015-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104805098-B |
| titleOfInvention | Come from peroxidase and the preparation of the degradable malachite green of mangrove sediment |
| abstract | Peroxidase and the preparation of the degradable malachite green of mangrove sediment are come from, is related to a kind of peroxidase, peroxidase gene comes from the grand genome sequence of mangrove sediment microorganism.Peroxidase gene is cloned, expression vector is inserted into, the recombinant expression carrier for carrying peroxidase is built；Recombinant expression carrier is transformed into E.coli BL21 (DE3)；Choose the fermented and cultured in culture medium of the positive colony after conversion in E.coli BL21 (DE3)；E.coli BL21 (DE3) cell after fermentation is collected by centrifugation, E.coli BL21 (DE3) cell is resuspended and is cracked in lysis buffer, centrifuges, supernatant is collected, then with being combined NiSO in advance 4 R10 Flammable mix, imidazoles elution, obtain peroxide Mgv rPOD.Gained peroxidase has notable degrading activity to malachite green. |
| priorityDate | 2015-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.