http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104805043-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-225 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate | 2015-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-104805043-B |
| titleOfInvention | Active Lactobacillus rhamnosus quick separating and method of counting in a kind of probiotic products |
| abstract | The present invention relates to active Lactobacillus rhamnosus quick separating and method of counting in a kind of probiotic products；Disclose applications of the Lactobacillus casei group pili subunit FimI in active Lactobacillus rhamnosus quick separating with counting.The pili subunit FimI of Lactobacillus casei group is obtained particular by escherichia expression system, immune mouse obtains polyclonal antibody after restructuring FimI ni-sepharose purifications；MRS flat board coatings, Anaerobic culturel are carried out after routinely being processed containing Lactobacillus rhamnosus sample；Bacterium colony transfer is carried out by solid phase carrier of nitrocellulose membrane, transfer film is developed the color after drying fixed, skimmed milk closing, washing, adding antibody incubation with DAB；According to the spot occurred on film to sample in active Lactobacillus rhamnosus count, and Lactobacillus rhamnosus quick separating is realized by speckle displacement.The inventive method is easy to operate effectively to be easy to promote the use of, and the separation identification and its quality evaluation for being adapted to Lactobacillus rhamnosus in probiotic products are used. |
| priorityDate | 2015-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.